一、中文名称

PG电子 Anti-DNA-RNA杂交抗体(S96)(RGK60001)

二、产品描述

在真核细胞中，DNA-RNA杂交体是一种普遍存在的自然现象，尤其在高转录活动位点的水平更高。作为一种非经典的核酸结构，这些杂交体具备转录调控的功能。研究表明，DNA-RNA的存在可能使某些基因位点更容易发生染色体断裂。DNA:RNA的形成会生成双链A/B中间构象，并在互补的被置换DNA链上提供第二个单链核酸结合蛋白的结合位点。此外，这些杂交体对DNA甲基转移酶的活性表现出抗性。研究还发现，DNA-RNA杂交体的形成与多种神经系统疾病密切相关，特定的DNA:RNA解旋酶senataxin（SETX）突变与显性青少年肌萎缩侧索硬化症4型及隐性眼动失协调2型存在关联。

三、产品详情

• 名称：PG电子 Anti-DNA-RNA杂交抗体(S96)(RGK60001)
• 货号：RGK60001
• 表达系统：哺乳动物细胞
• 种属反应性：通用
• 宿主：小鼠
• 同种型：IgG2a, kappa
• 克隆类型：单克隆
• 靶标：DNA-RNA杂交体
• 浓度：0.58 mg/ml
• 内毒素水平：请联系实验室获取此信息
• 纯度：95%（基于SDS-PAGE判定）
• 纯化方式：通过Protein A/G从细胞培养上清液中纯化
• 克隆号：S96
• 应用：ChIP, ChIP-seq, DB, EMSA, FISH, ICC, IF, IP, SPR
• 状态：液态
• 保存溶液：0.01M PBS, pH 7.4
• 稳定性与存储：使用手动解冻冰箱，避免反复冻融。短期存储在4°C (1-2周)，长期存储在-20°C可达12个月，或在-80°C保存。

四、参考文献

• Recognition of RNA by the S96 antibody creates pervasive artifacts when imaging RNA:DNA hybrids. PMID: 33830170
• Immunoprecipitation of DNA:RNA Hybrids Using the S96 Antibody. PMID: 32681514
• High-resolution, strand-specific R-loop mapping via S96-based DNA-RNA immunoprecipitation and high-throughput sequencing. PMID: 31053798
• RNA/DNA Hybrid Interactome Identifies DXH9 as a Molecular Player in Transcriptional Termination and R-Loop-Associated DNA Damage. PMID: 29742442
• The monoclonal S96 antibody exhibits highly variable binding affinities towards different R-loop sequences. PMID: 28594954
• Structural basis of R-loop recognition by the S96 monoclonal antibody. PMID: 35347133
• Best practices for the visualization, mapping, and manipulation of R-loops. PMID: 33411340
• Catalytically inactive, purified RNase H1: A specific and sensitive probe for RNA-DNA hybrid imaging. PMID: 34232287
• The Affinity of the S96 Antibody for Double-Stranded RNAs Impacts the Accurate Mapping of R-Loops in Fission Yeast. PMID: 29289567
• The sub-nanomolar binding of DNA-RNA hybrids by the single-chain Fv fragment of antibody S96. PMID: 23784994
• Modifications and interactions at the R-loop. PMID: 32961406
• BRCA2 prevents R-loop accumulation and associates with TREX-2 mRNA export factor PCID2. PMID: 24896180
• R-Loop Analysis by Dot-Blot. PMID: 33554969
• Amplification-Free and Mix-and-Read Analysis of Multiplexed MicroRNAs on a Single Plasmonic Microbead. PMID: 34324345
• New insight into the biology of R-loops. PMID: 32516653
• Detection of Telomeric DNA:RNA Hybrids Using TeloDRIP-qPCR. PMID: 33371452
• Ultra-deep Coverage Single-molecule R-loop Footprinting Reveals Principles of R-loop Formation. PMID: 32105733
• Complexities due to single-stranded RNA during antibody detection of genomic RNA:DNA hybrids. PMID: 25890199
• Development of electrochemical biosensors for simultaneous multiplex detection of microRNA for breast cancer screening. PMID: 34495394

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